Phosphorylation of histone 2AX (gamma H2AX) protein is involved in regulating DNA damage repair and apoptosis signaling. Adriamycin or CFM-4.16 treatments inhibited cell growth and caused elevated CARP-1 and gamma H2AX in human breast (HBC) and cervical cancer (HeLa) cells.

Rabbit Polyclonal Anti-gamma H2AX [p Ser139] Antibody DNA Double-strand break marker cited in 104 publications. Validated: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ChIP, KO.

H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation (5-8). H2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10). DOI: 10.1139/o03-042. Abstract.

3 reviews. Compare Anti-H2AX Antibody Products from Cell Signaling Technology from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. alexa-fluor-647-gamma-h2ax-phospho-s139-antibody-ep8542y-ab195189.pdf Send me a copy of this email I agree to the terms and conditions . Cell Signaling Technology, Inc Proper citation Cell Signaling Technology Cat#9718, RRID:AB_2118009 Product name Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb Provider product page Cell Signaling Technology, Inc - 9718 Antibody type Monoclonal Reactivity … Simple Western: gamma H2AX [p Ser139] Antibody [NB100-384] - Simple Western lane view shows a specific band for gamma H2AX in 0.2 mg/ml of Jurkat lysate(s). Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4).

H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from cellular response to gamma radiation • spermatogenesis γH2AX is a sensitive target for looking at DSBs in cells. The γH2AX signal is a

signaling and insulin resistance: evidence from mice and auditory HEI-OC1 cells. Quantitative γ-H2AX immunofluorescence method for DNA double-strand Radiation Therapy Model, Antioxidants & Redox SignalingVol.

2021-04-18 · A model is proposed for non-targeted (bystander) signaling leading to gamma H2AX foci induction in irradiated primary astrocytes and glioma cells. Genotoxic responses to ultraviolet radiation, like increase in the phosphorylation of histone H2AX, induction of p53 protein and binding of this protein to its DNA consensus sequence, were all decreased on the restrictive substrate.

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post Materials and methods: Microscopic scoring of the gamma H2AX foci was used to evaluate the DNA-double-strand-break repair capacity in Cytoskeletal Signaling inhibitor from Ataxia-Telangiectasia (A-T) patients derived lymphoblastoid cell lines (LCL) and T-lymphocytes isolated from … Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).

The gamma-H2AX signal and the comet signal were lower in neutrophils compared to monocytes and T-lymphocytes. Here we show that inactivation of the SWI/SNF complexes and downregulation of the catalytic core subunits of the complexes both result in inefficient DNA double-strand break (DSB) repair and increased DNA damage sensitivity as well as a large defect in H2AX phosphorylation (gamma-H2AX) and nuclear focus formation after DNA damage. The results suggest gamma-H2AX is a useful adjunct in diagnosis of metastatic RCC when RCC-Ma is negative and in higher grade RCC, which are often a diagnostic challenge. A nuclear pattern of staining of gamma-H2AX has a comparable sensitivity with RCC-Ma, and the interpretation is easier and more reliable. ab11174 at 1/1000 staining human HeLa cells by ICC/IF.
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Predicted band size: 15 kDa. 15% 1. Haematologica. 2010 Dec;95(12):1996-2004. doi: 10.3324/haematol.2010.026492.

Gamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. The kit provides sufficient reagents for up to 100 stainings in 96- well plate 2/14/2021 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (#9718) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=9718&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 I mmunohis t oc hemis t ry (P araf f in) 1: 480 Cells were cultured in DMEM Ham's F-12 containing 5% fetal calf serum (FCS) in a humidified atmosphere with 7% CO 2 at 37°C. Cells were checked before experimental use for mycoplasma contamination. Cells were treated with increasing concentrations of genotoxicants and nongenotoxicants.
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2013-11-01 · H2AX is a member of the histone protein H2A family; the other two members are H2A1-H2A2 and H2AZ . γH2AX foci formation at DSB sites occurs rapidly (within minutes) and is highly conserved from yeast to humans . γH2AX can recruit other DSB signaling and repair factors such as MDC1, 53BP1 and the MRN complex to the damage site, forming nuclear ionizing radiation-induced foci (IRIF) , , .

Secondary antibody Mouse … 1/17/2021 Phospho-Histone H2A.X (Ser139) Antibody (#2577) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=2577&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). Histone H2AX, Histone H2A.X, H2AX_HUMAN. UniProt Code History.

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Cell Signaling Technology, Inc Proper citation Cell Signaling Technology Cat#9718, RRID:AB_2118009 Product name Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb Provider product page Cell Signaling Technology, Inc - 9718 Antibody type Monoclonal Reactivity …

2016-03-31 · The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions.